Chromatin modification of Apaf-1 restricts the apoptotic pathway in mature neurons

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Chromatin modification of Apaf-1 restricts the apoptotic pathway in mature neurons

Although apoptosis has been extensively studied in developing neurons, the dynamic changes in this pathway after neuronal maturation remain largely unexplored. We show that as neurons mature, cytochrome c- mediated apoptosis progresses from inhibitor of apoptosis protein-dependent to -independent regulation because of a complete loss of Apaf-1 expression. However, after DNA damage, mature neuro...

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APAF 1 ( Apoptotic protease activating factor 1 )

Five isoforms of APAF1 cDNA have been identified in Homo sapiens: The original APAF1 (also called Apaf-1S) is 3585 bp long (it contains 12 WD40 repeats); The APAF1-1M isoform (3618 bp) contains an insertion of 33 nt after position 295 of the first published sequence (11 aa insertion GKDSVSGITSY between aa 98 and 99; it also contains 12 WD40 repeats); The APAF1XS isoform (3516 bp) contains the s...

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Apoptotic protease-activating factor 1 (Apaf-1) as a liable gene for spontaneous mutations in vitro

The apoptotic protease-activating factor 1 (Apaf-1) receives the death signal in the intrinsic ormitochondrial pathway of apoptosis. Upon the releasing of cytochrome c from theintermembrane space of mitochondria and binding to Apaf-1 molecules, a heptamericapoptosome complex is formed and triggers the downstream cascade of caspases. Here, for thefirst time we present spontaneous mutations and r...

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Subcellular localization of Apaf-1 in apoptotic rat pituitary cells.

A key step in the intrinsic apoptotic pathway is the assembly of the apoptosome complex. The apoptosome components are well known; however, the physiology of the assembly of the apoptosome complex at the cellular level is still poorly defined. The aim of this work was to study the subcellular distribution of the apoptosome scaffold protein apoptotic protease-activating factor 1 (Apaf-1) before ...

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apoptotic protease-activating factor 1 (apaf-1) as a liable gene for spontaneous mutations in vitro

the apoptotic protease-activating factor 1 (apaf-1) receives the death signal in the intrinsic ormitochondrial pathway of apoptosis. upon the releasing of cytochrome c from theintermembrane space of mitochondria and binding to apaf-1 molecules, a heptamericapoptosome complex is formed and triggers the downstream cascade of caspases. here, for thefirst time we present spontaneous mutations and r...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2007

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.200708086